Use of melatonin to prevent selenite-induced cataract formation in rat eyes


Yağcı R., Aydin B. , Erdurmuş M., Karadag R., GUEREL A., Durmuş M., ...More

CURRENT EYE RESEARCH, vol.31, no.10, pp.845-850, 2006 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 10
  • Publication Date: 2006
  • Doi Number: 10.1080/02713680600899663
  • Title of Journal : CURRENT EYE RESEARCH
  • Page Numbers: pp.845-850

Abstract

Purpose: To evaluate effects of melatonin on sodium selenite-induced cataract formation. Methods: Twenty-three Sprague-Dawley rat pups were randomized into three groups. Group 1( n = 9), injected with selenite (s.c.) on postpartum day 10; group 2 ( n = 7), injected with selenite (s.c.) on day 10 plus melatonin (i.p.) on days 8 - 15; group 3 ( n = 7), saline-injected controls. Development of cataract was assessed weekly under a dissection microscope. Rat lenses and serums were analyzed for antioxidant enzymes superoxide dismutase ( SOD) and catalase ( CAT); oxidative stress indicators xanthine oxidase (XO) and malondialdehyde (MDA), a marker of lipid peroxidation; and protein carbonyl ( PC), a marker of protein oxidation. Results: Significant differences ( p < 0.05) were seen in cataract development by the three groups. All rats developed dense nuclear cataract in group 1. Dense nuclear cataract was not observed in group 2: five of seven rats developed minor cataracts, while the other two had clear lenses. In control rats ( group 3), all lenses remained clear. In selenite group ( group 1), lens and serum levels of MDA, PC, and XO were significantly higher and levels of SOD and CAT were significantly lower than those in control group ( p < 0.001). In selenite + melatonin group ( group 2), lens and serum levels of MDA, PC, and XO significantly decreased and levels of SOD and CAT significantly increased when compared with selenite group. Conclusions: Studies with the rat selenite cataract model strongly support the activity of melatonin as an endogenous antioxidant and anticataract agent.