Background and Purpose: The purpose of this study was to adapt and assess cultural and psychometric properties of the Adolescent Life Style Profile (ALP-R2) in Turkish adolescents. Methods: The sample of the study consisted of 945 students aged 12-18 years. Data were collected using the Turkish translation of the ALP-R2. Psychometric testing of the ALP-R2 included internal consistency, item-total correlation, test-retest correlation, and construct validity with confirmatory factor analysis (CFA). Results: The total Cronbach's a coefficient was .88, and the subscales ranged from .55 to .76. The CFA results showed that the structure of the model/scale has an acceptable goodness of fit. Conclusion: The study shows that the Turkish version of the ALP-R2 is a reliable and valid scale that can be used to assess healthy lifestyle domains in Turkish adolescents.