Correlation Between The Ultrasound-Guided Thyroid Fine-Needle AspirationCytology With Molecular Testing And Surgical HistopathologyResults

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Selendir H., Kır G., Söylemez T., Girgin R., Özbay N., Özen F., ...More

31th European Congress of Pathology, Nice, France, 7 September - 11 November 2019, vol.31, no.1, pp.3-9

  • Publication Type: Conference Paper / Full Text
  • Volume: 31
  • City: Nice
  • Country: France
  • Page Numbers: pp.3-9


Background & Objectives: Molecular testing has been proposed to

refine the assessment of cancer risk in thyroid nodules with indeterminate

cytology. The aim of this study is to compare the genotypic alterations for

BRAF, NRAS, and KRAS mutations of FNA samples in indeterminate

and malignant category with subsequent histology of surgical specimens

in our routine practices.

Methods: We reviewed 150 cases diagnosed as atypical by ultrasoundguided

thyroid FNA cytology on the basis of the Bethesda system who

had undergone molecular testing. FNA samples were tested for BRAF,

NRAS, and KRAS point mutations by real-time polymerase chain reaction

(RT-PCR). Out of these 150 cases, 71 had undergone surgical procedure

and histopathology results,were compared with both cytology and

molecular status. According to the final pathological results, we divided

the cases, as benign and malign groups.

Results: According to the Bethesda System the 71 cases were distributed as

13 AUS/FLUS, 24 FN/SFN, 13 SM, and 21 malign categories. Of the 71

cases of atypical cytology, 48 cases (67.6 %) were diagnosed as malignant,

and 23 cases (32.4 %) were diagnosed as benign at surgical specimens. In

71 nodules, 37 samples (52.1%) had point mutations. Overall positive

predictive value of cytology evaluation was 67.6 % and overall positive

predictive value of cytology and molecular testing was 89.2 % (p=0.004).

Conclusion: The addition of molecular testing to FNA cytology may

increase the positive predictive value of cytology.