Background: Currently, there are various antiseptics used for cleaning the skin before surgery, but there is no standard procedure in practice. Chlorhexidine and povidone-iodine are the most preferred compounds among antiseptics. Both are proved to be safe and effective for skin disinfection. In this study. our aim was to investigate the combined effects of chlorhexidine and povidone-iodine oil the skin's flora before neurosurgical intervention, consecutively.