Lack of association with TNF-alpha-308 promoter polymorphism in patients with vitiligo


Yazici A. C. , Erdal M. E. , Kaya T. I. , Ikizoglu G., Savasoglu K., Camdeviren H. , et al.

ARCHIVES OF DERMATOLOGICAL RESEARCH, cilt.298, ss.46-49, 2006 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 298 Konu: 1
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1007/s00403-006-0664-2
  • Dergi Adı: ARCHIVES OF DERMATOLOGICAL RESEARCH
  • Sayfa Sayısı: ss.46-49

Özet

Vitiligo is an acquired depigmentary disorder of the skin, characterized by incomplete penetrance, multiple susceptibility loci and genetic heterogeneity. An immunologic hypothesis is currently advanced as a possible pathogenesis of vitiligo. The cytokines have an important role in pathogenesis of autoimmunity in which tumor necrosis factor-alpha (TNF-alpha), a paracrine inhibitor of melanocytes, is especially important. Several single-nucleotide polymorphisms (SNP) have been identified in the human TNF gene promoter. The polymorphism at position -308 (TNF-308), which involves substituting G for A and designing the AA genotype, leads to a higher rate of TNF gene transcription than the wild-type GG genotype in in vitro expression studies. It has also been linked to increased susceptibility to several chronic metabolic, degenerative, inflammatory and autoimmune diseases. Therefore, we investigated the TNF-alpha-308 SNP in patients with vitiligo. We examined 61 patients with vitiligo. Healthy age-, ethnically- and sex-matched individuals (n = 123) served as controls. Polymerase chain reaction amplification was used for analysis of the polymorphism at position -308 in promoter of TNF-alpha gene. We found that the distribution of TNF-alpha genotypes in vitiligo patients did not differ from that in control subjects (P > 0.05). Moreover, there was no association between TNF-alpha genotypes and types of vitiligo. In conclusion, we suggest that TNF-alpha-308 SNP is not a genetic risk factor for vitiligo susceptibility.