This study analysed the effect of needle irrigation (NI), passive ultrasonic irrigation (PUI), EDDY and the use of the XP-endo Finisher (XPF) on the removal of modified triple antibiotic paste (mTAP) from artificially created grooves in root canals. Forty-eight maxillary incisors were prepared up to size 50, with a standard groove on one surface of the root canal wall and filled with mTAP. Re-attached roots were analysed based on the activation techniques applied for 180 s with 5 ml 3% sodium hypochlorite. The results showed that EDDY was more effective at removing mTAP than PUI, XPF and needle irrigation (P < 0.05), whereas XPF and PUI were significantly more effective than needle irrigation. (P < 0.05). No significant difference in mTAP removal was observed between XPF and PUI (P > 0.05). These findings suggest that the removal of mTAP can be more efficiently achieved with EDDY than with XPF, PUI or NI.