Aim: Entamoeba histolytica (E. histolytica) and E. dispar are morphologically identic but they are genetically distinct according to their immunological, molecular biological and biochemical characteristics. E. histolytica is the invasive species that leads to amoebic colitis and liver abscesses and E. dispar which exerts non-invasive features. Especially laboratory technicans who are not experienced enough can confuse the E. histolytica/dispar with leukocytes, macrophages and other trophozoites under direct microscopy. However, since E. histolytica is the reason for amoebic dysentery cases, certain and accurate diagnosis is very crutial in order to plan the treatment. In our study, we have used the ELISA technique which depends on searching the E. histolytica adhesin antigen in stool to bring this technique for the routine detection and diagnosis.