Purpose: To evaluate the tooth discoloration effect of the daily use protocol of six different mouthrinses. Methods: 70 extracted caries -free central incisors were divided into seven groups (n=10/per group): Group 1: Curasept Anti Discoloration System; Group 2: CB12; Group 3: Listerine Zero; Group 4: Listerine Cool Mint; Group 5: Colgate Plax; Group 6: Kloroben. The teeth were exposed to the mouthrinse for 2 minutes daily for 3 weeks. Color measurement was performed four times: at the beginning of the study, and after 1, 2 and 3 weeks, with a digital spectrophotometer (Vita Easyshade Advanced). Color changes (Delta E) were calculated and ANOVA and post hoc Tukey's test were used for statistical analysis. Results: Although there was no statistically significant difference between the Colgate Plax and control groups, both groups had a significant difference from the other groups within all Delta E1, Delta E2 and Delta E3 values.