Quantitative immunohistochemical analysis of nitric oxide synthases and apoptosis regulator proteins in the fetal rat brain following maternal uterine artery ligation

UYSAL A., ÖKTEM G., YILMAZ Ö., Uslu S., AKTUĞ H., Yurtseven M. E.

INTERNATIONAL JOURNAL OF NEUROSCIENCE, vol.118, no.6, pp.891-901, 2008 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 118 Issue: 6
  • Publication Date: 2008
  • Doi Number: 10.1080/00207450701769364
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.891-901


The purpose of this study was to determine the relation between nitric oxide synthases (calcium-independent NOS and calcium-dependent eNOS) and apoptosis regulator proteins (anti-apoptotic Bcl-2, pro-apoptotic p53) of fetal rat brain in experimental intrauterine growth retardation (IUGR) model via quantitative immunohistochemistry. Cortical zone of parietal cerebral cortex and ventricular zone of third ventricle were studied following bilateral uterine artery ligation on gestational day 18. Significant increase in NOS immunoreactivity was determined in parietal cerebral cortex and ventricular zones as eNOS immunoreactivity increased in ventricular zone of IUGR group. Bcl-2 expression was significantly decreased in ventricular zone; whereas cortical zone of IUGR group expressed p53 immunoreactivity.