Effects of iloprost on bleomycin-induced pulmonary fibrosis in rats compared with methyl-prednisolone


AYTEMUR Z. A. , HACIEVLİYAGİL S. S. , Iraz M., ŞAMDANCI E., ÖZEROL E., KUKU İ., et al.

REVISTA PORTUGUESA DE PNEUMOLOGIA, cilt.18, ss.272-277, 2012 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 18 Konu: 6
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1016/j.rppneu.2012.04.010
  • Dergi Adı: REVISTA PORTUGUESA DE PNEUMOLOGIA
  • Sayfa Sayısı: ss.272-277

Özet

Objective: Prostacyclin (PGI2) has been shown to inhibit the expression of pro-inflammatory and pro-fibrotic mediators in pulmonary fibrosis. In this study, we aimed to test the preventive effects of intraperitoneally administered iloprost, a stable PGI2 analog, on bleomycin-induced pulmonary fibrosis in rats and to compare the effects of iloprost with the effects of methylprednisolone, a traditional therapy.

Objective: Prostacyclin (PGI2) has been shown to inhibit the expression of pro-inflammatory and pro-fibrotic mediators in pulmonary fibrosis. In this study, we aimed to test the preventive effects of intraperitoneally administered iloprost, a stable PGI2 analog, on bleomycin-induced pulmonary fibrosis in rats and to compare the effects of iloprost with the effects of methylprednisolone, a traditional therapy. 

Methods: Rats were randomly allocated into four groups: 1. Saline alone (n = 6); 2. Bleomycin + placebo (n = 7); 3. Bleomycin + methyl-prednisolone (n = 7); 4. Bleomycin + iloprost (n = 7). Fibrotic changes in the lungs were demonstrated by analyzing the cellular composition of bronchoalveolar lavage fluid, histological evaluation and lung hydroxyproline content. 

Results: Fibrosis was made in the lungs of rats by bleomycin experimentally. Fibrosis scores in the methyl-prednisolone and the iloprost groups were significantly lower than in the placebo group (p < 0.05). Furthermore, the score of the iloprost group was significantly lower than the score of the methyl-prednisolone group. The hydroxyproline content was significantly less in the methyl-prednisolone and the iloprost groups (p < 0.05). In the placebo group, the neutrophil percentage in bronchoalveolar lavage was significantly higher than in the other groups, whereas the macrophage percentage in placebo group was significantly lower (p < 0.05). 

Conclusion: Iloprost has protective effect on the pulmonary fibrosis induced by bleomycin and it may be more effective in decreasing fibrotic changes than methyl-prednisolone. (C) 2011 Sociedade Portuguesa de Pneumologia. Published by Elsevier Espana, S. L. All rights reserved.