Characterization of Cloned Cattle Obtained by Nuclear Transfer and Their Offspring with mtDNA and Microsatellite Markers


Tuten Sevim E., Ozdil F. , Ozkan Unal E., Arat S.

JOURNAL OF TEKIRDAG AGRICULTURE FACULTY-TEKIRDAG ZIRAAT FAKULTESI DERGISI, vol.14, no.2, pp.150-157, 2017 (Journal Indexed in ESCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 14 Issue: 2
  • Publication Date: 2017
  • Title of Journal : JOURNAL OF TEKIRDAG AGRICULTURE FACULTY-TEKIRDAG ZIRAAT FAKULTESI DERGISI
  • Page Numbers: pp.150-157
  • Keywords: Cloning, Grey Cattle, mtDNA D-Loop, microsatellite, SOMATIC-CELLS, FETAL, ADULT, CLONING, CALVES, CALF, TERM

Abstract

Cloning technology is based on the principle of turning back to embryonic period by reprogramming of that an adult cell nucleus was put into the oocyte cell. However, affecting factors of this reprogramming has not been clarified completely. Therefore, cloning technology can be made more understandable and controllable with a more detailed manner identifying of obtained clones and their offspring. The purpose of the study is to characterize the clones and their generations molecularly to reveal whether it is possible or not that creation of a normal race population through clones which acquired as a result of cloning individuals of one race by using ovular source which belongs to a different race, and thus to try to understand better that possible potential of this technology to reinstate an extincted race. For this purpose, 5 clone grey cattles (1 male, 4 female) and their offsprings (2 male, 4 female) which generated with TUBITAK TOVAG-104O360- project had been used as material. First of all 10 microsatellite markers were used to test the clones, their offspring and donor cells which were used for producing the clones. It has been confirmed that the clones are the copy of donor cells and all offspring belong to the clones and by sequencing of D-Loop region of mtDNA, it has been determined that the origin of mtDNA from clones are eggs and therefore are different than donor cell. In addition the presence of mtDNA difference has been observed in offspring of the clones.