Antioxidative status and lipid peroxidation in kidney tissue of rats fed with vitamin B-6-deficient diet

Keleş M., Al B., Gümüştekin K., DEMİRCAN B., Özbey İ., Akyüz M., ...More

RENAL FAILURE, vol.32, no.5, pp.618-622, 2010 (Peer-Reviewed Journal) identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 5
  • Publication Date: 2010
  • Doi Number: 10.3109/0886022x.2010.481737
  • Journal Name: RENAL FAILURE
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.618-622


Introduction: The aim of this study was to evaluate lipid peroxidation (LP) and free radical scavenging enzyme activities in kidney tissue of vitamin B-6-deficient rats. Material and Methods: The rats were divided into control and vitamin B-6-deficient groups. After 4 weeks of feeding, animals in all groups were anesthetized by thiopental sodium (50 mg/kg). Thoraces were opened, 2 mL blood samples were taken from aortas, then the rats were killed by cervical dislocation, and kidney tissues were removed. Biochemical measurements in kidney tissue were carried out using a spectrophotometer. Results: Total superoxide scavenger activity (TSSA), nonenzymatic superoxide scavenger activity (NSSA), superoxide dismutase (SOD) activities, and antioxidant potential (AOP) values in the vitamin B-6-deficient group were significantly lower than those of the control group, whereas glutathione peroxidase (GSH-Px), glutathione reductase (GRD), glutathione-S-transferase (GST) activities, and malondialdehyde (MDA) level were significantly higher than those of the control group (p < 0.05). Discussion: The results show that vitamin B-6 deficiency causes an attenuation in antioxidant defense system and an increase in oxidative stress in kidney tissue of rats.