Antioxidative status and lipid peroxidation in kidney tissue of rats fed with vitamin B-6-deficient diet


Keleş M., Al B., Gümüştekin K., DEMİRCAN B. , Özbey İ., Akyüz M., et al.

RENAL FAILURE, cilt.32, ss.618-622, 2010 (SCI İndekslerine Giren Dergi) identifier

  • Cilt numarası: 32 Konu: 5
  • Basım Tarihi: 2010
  • Doi Numarası: 10.3109/0886022x.2010.481737
  • Dergi Adı: RENAL FAILURE
  • Sayfa Sayısı: ss.618-622

Özet

Introduction: The aim of this study was to evaluate lipid peroxidation (LP) and free radical scavenging enzyme activities in kidney tissue of vitamin B-6-deficient rats. Material and Methods: The rats were divided into control and vitamin B-6-deficient groups. After 4 weeks of feeding, animals in all groups were anesthetized by thiopental sodium (50 mg/kg). Thoraces were opened, 2 mL blood samples were taken from aortas, then the rats were killed by cervical dislocation, and kidney tissues were removed. Biochemical measurements in kidney tissue were carried out using a spectrophotometer. Results: Total superoxide scavenger activity (TSSA), nonenzymatic superoxide scavenger activity (NSSA), superoxide dismutase (SOD) activities, and antioxidant potential (AOP) values in the vitamin B-6-deficient group were significantly lower than those of the control group, whereas glutathione peroxidase (GSH-Px), glutathione reductase (GRD), glutathione-S-transferase (GST) activities, and malondialdehyde (MDA) level were significantly higher than those of the control group (p < 0.05). Discussion: The results show that vitamin B-6 deficiency causes an attenuation in antioxidant defense system and an increase in oxidative stress in kidney tissue of rats.