Antioxidant and anti-apoptotic effects of onion (Allium cepa) extract on doxorubicin-induced cardiotoxicity in rats


Alpsoy S., Aktaş C., Uygur R., Topçu B., KANTER M., Erboğa M., ...More

JOURNAL OF APPLIED TOXICOLOGY, vol.33, no.3, pp.202-208, 2013 (Journal Indexed in SCI) identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 3
  • Publication Date: 2013
  • Doi Number: 10.1002/jat.1738
  • Title of Journal : JOURNAL OF APPLIED TOXICOLOGY
  • Page Numbers: pp.202-208

Abstract

The aim of this study was to investigate the antioxidant and anti-apoptotic effects of onion (Allium cepa) extracts (ACE) on doxorubicin (DOX)-induced cardiotoxicity. The rats in the ACE-pretreated group were given a daily dose of 1 ml ACE for 14 days. To induce cardiotoxicity, DOX (30 mg kg-1 body weight) was injected intraperitoneally by a single dose and the rats were sacrificed after 48 h. To date, no such studies have been performed on the cardioprotective and anti-apoptotic potential of ACE on DOX-induced cardiotoxicity. Our data indicate a significant reduction in the activity of in situ identification of apoptosis using terminal dUTP nick end-labeling in cardiomyocytes of the DOX-treated group with ACE therapy. The DOX-treated with ACE groups showed a significant decrease in malondialdehyde levels, and increased activities of superoxide dismutase, glutathione and glutathione peroxidase in comparison with the DOX-treated group. Creatine kinase, creatine kinase MB, lactate dehydrogenase activities and cardiac troponin I levels were significantly decreased in the DOX + ACE group in comparison with the DOX group. These biochemical and histological disturbances were effectively attenuated on pretreatment with ACE. The present study showed that ACE may be a suitable cardioprotector against toxic effects of DOX. Copyright (C) 2011 John Wiley & Sons, Ltd.