DENSpm overcame Bcl-2 mediated resistance against Paclitaxel treatment in MCF-7 breast cancer cells via activating polyamine catabolic machinery


Akyol Z., ÇOKER GÜRKAN A., Arisan E. D. , Obakan-Yerlikaya P. O. , Palavan-Unsal N.

BIOMEDICINE & PHARMACOTHERAPY, vol.84, pp.2029-2041, 2016 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 84
  • Publication Date: 2016
  • Doi Number: 10.1016/j.biopha.2016.11.016
  • Title of Journal : BIOMEDICINE & PHARMACOTHERAPY
  • Page Numbers: pp.2029-2041
  • Keywords: Paclitaxel, DENSpm, Apoptosis, Polyamine, Breast cancer, TAXOL-INDUCED APOPTOSIS, HUMAN-MELANOMA CELLS, PHASE-III TRIAL, SPERMIDINE/SPERMINE N-1-ACETYLTRANSFERASE, ANALOG N-1,N-11-DIETHYLNORSPERMINE, CARCINOMA-CELLS, LUNG-CANCER, KINASE ACTIVATION, COLON-CARCINOMA, CHEMOTHERAPY

Abstract

Purpose: The Bcl-2 mediated resistance is one of the most critical obstacle in cancer therapy. Conventional chemotherapeutics such as Paclitaxel, a commonly used in the treatment of metastatic breast cancer, is not sufficient to overcome Bcl-2 mediated drug resistance mechanism. Thus, combinational drug regimes are favored by researchers to overcome resistance phenotype against drugs. N1, N11-diethylnorspermine (DENSpm), a polyamine analogue, which is a promising drug candidate induced-cell cycle arrest and apoptosis in various cancer cells such as prostate, melanoma, colon and breast cancer cells via activated polyamine catabolism and reactive oxygen generation. Recent studies indicated the potential therapeutic role of DENSpm in phase I and II trials in breast cancer cases. Although the molecular targets of Paclitaxel in apoptotic cell death mechanism is well documented, the therapeutic effect of DENSpm and Paclitaxel in breast cancer cells has not been investigated yet. In this study, our aim was to determine the time dependent effect of DENSpm and Paclitaxel on apoptotic cell death via determination of polyamine metabolism related targets in wt and Bcl-2 overexpressing MCF-7 breast cancer cells.