Effects of Dexamethasone Exposure on Neural Crest Cells and Primary and Secondary Neurulation in Chick Embryos

USLU S., Emon S. T. , ALACA N., Kocaturk D. C. , Uslu U., UYSAL A.

TURKISH NEUROSURGERY, vol.32, no.3, pp.412-419, 2022 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 3
  • Publication Date: 2022
  • Doi Number: 10.5137/1019-5149.jtn.34904-21.2
  • Journal Name: TURKISH NEUROSURGERY
  • Journal Indexes: Science Citation Index Expanded, Scopus, EMBASE, MEDLINE
  • Page Numbers: pp.412-419
  • Keywords: Primary neurulation, Secondary neurulation, Neural crest cells, Dexamethasone, Chick embryo, EXTRACELLULAR-MATRIX, PLATE BORDER, TUBE, MECHANISMS, HAMBURGER, ORGANIZER

Abstract

AIM: To evaluate the effects of dexamethasone (Dex) treatment on neural crest cells and primary and secondary neurulation in chick embryos. MATERIAL and METHODS: Sixty fertilized eggs with an average weight of 65 ?? 2 g were incubated in 60%???70% humidity at 37.2??C ?? 0.1??C. After 26 hours of incubation, the control group (n=12) received 0.1 mg/kg physiologic saline (S), group 1 (n=12) received 0.1 mg/kg Dex, group 2 (n=12) received 1 mg/kg Dex, and group 3 (n=12) received 5 mg/kg Dex into each embryonic disc. The eggs were incubated until Hamburger???Hamilton stage (HH) 15, HH18, and HH20. Then, the embryos were dissected and evaluated both macroscopically and microscopically. RESULTS: The mortality rate in the control group, group 1, and groups 2 and 3 was 27%, 48%, and 100%, respectively. The neural tube thicknesses in group 1 significantly increased in HH 15 and HH20 (p<0.05). The mitosis number in group 1 significantly decreased in each stage (p<0.05). Wnt-1 expression was significantly lower in group 1 in HH15 (p<0.05) and HH18 (p 0.05), but there was no significant difference in HH20 (p 0.05). Fibroblast growth factor (FGF) expression was significantly lower in group 1 in HH15 (p<0.05). The expression of N-cadherin was significantly higher in group 1 in HH20 (p<0.05). Fibronectin expression CONCLUSION: Although the Dex treatment did not result in neural tube closure defect, the mortality rates and neural tube thicknesses increased, whereas mitotic activation and Wnt-1 and FGF signal pathways reduced in some stages.