MCM2019 14th MULTINATIONAL CONGRESS ON MICROSCOPY SEPTEMBER 15–20, 2019 IN BELGRADE, SERBIA, Belgrade, Serbia And Montenegro, 15 - 20 September 2019, pp.193-194
Objectives: Adipokinetic hormones (AKHs) are small stress peptides found in insects that
regulate metabolic responses to stress (1). Insect anti-stress responses, including those induced
by insecticides, are controlled by adipokinetic hormones. AKHs are synthesized by
neurosecretory cells of the corpora cardiaca of insects. However, presence of AKHs had also
been reported the cells within the brain, although their roles are not clear (2).
Astrocytes have been shown to play crucial roles in regulating both normal and disease states.
Astrocyte dysfunction has been related to neuroautoimmune diseases, neoplasms and epilepsy
(3-5). Brain-derived neurotrophic factor (BDNF) is one of the major neurotrophic factors
produced by astrocytes to maintain the development and survival of neurons in the brain, and
have recently been shown to modulate homeostasis of neuroinflammation. BDNF plays vitally
important roles in neural development and plasticity in both health and disease. Glial cells are
able to store and release BDNF (6) and it has been suggested that glial dysfunction may
contribute to the patho-physiology of schizophrenia (7-9). The aim of this study was to
investigate the effects of Anax imperator AKH (Ani-AKH) on the astrocyte-like model C6 rat
glioma cell line, using BDNF immunohistochemistry stainings.
The C6 rat glioma cell line was cultured in Dulbecco's modified Eagle's medium-F12 cell
culture medium supplemented with 10% heat-inactivated fetal calf serum. Ani-AKH was
applied to the cultured cells at the concentrations of 5, 10 and 50 µg/ml for 24 hours and
evaluated the BDNF staining density.
Results: BDNF staining density was significantly increased in the Anax imperator AKH (AniAKH) 5, 10 and 50 µg/ml groups than the control group. BDNF staining density was higher in
the 50 µg/ml Ani-AKH group than in the 5 and 10- µg/ml Ani-AKH groups.
Discussion: Our findings suggest that Ani-AKH has a potential increased the expression of
BDNF on C6 rat glioma cell line. The further studies will be required working with additional
cell lines, different doses and experimental design