The investigation of Chlamydophila pneumoniae in patients with Multiple sclerosis


VAHABOĞLU H.

INTERNATIONAL JOURNAL OF NEUROSCIENCE, cilt.117, ss.409-415, 2007 (SCI İndekslerine Giren Dergi) identifier

  • Cilt numarası: 117 Konu: 3
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1080/00207450600773541
  • Dergi Adı: INTERNATIONAL JOURNAL OF NEUROSCIENCE
  • Sayfa Sayıları: ss.409-415

Özet

Totally 32 cerebrospinal fluid samples from Multiple sclerosis (MS) patients were collected. DNA was extracted by High Pure PCR Template Preparation Kit. Two genomic segments, outer membrane protein genes ompA and omp9, were targeted for the detection of C. pneumoniae DNA in the samples by PCR tests. To detect ompA, a nested-PCR assay was designed, whereas for omp9, a PCR-Enyzme immunoassay (PCR-EIA) depending on streptavidin-biotin capture and dig detection of the PCR products was performed. C. pneumoniae DNA was not detected by each assays in patient samples.