Inhibitory effects of selected pesticides on peroxidases purified by affinity chromatography


KÖKSAL Z., Kalin R., GÜLÇİN İ., ÖZDEMİR H.

INTERNATIONAL JOURNAL OF FOOD PROPERTIES, vol.21, no.1, pp.385-394, 2018 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 1
  • Publication Date: 2018
  • Doi Number: 10.1080/10942912.2018.1424197
  • Journal Name: INTERNATIONAL JOURNAL OF FOOD PROPERTIES
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.385-394

Abstract

The objective of this study was to determine the in vitro inhibition effects of seven commonly used pesticides including 2,4-d-acid dimethylamine, fenoxaprop-p-ethyl, glyphosate isopropylamine, haloxyfop-p-methyl, cypermethrin, -cyhalothrin, and dichlorvos on the peroxidase purified from turnip (Brassica rapa L.) and black radish (Raphanus sativus L.) using 4-amino benzohydrazide affinity column chromatography. The purification factors for the turnip and black radish peroxidases were found to be 263.29-fold (with a yield of 12.89%) and 36.20-fold (with a yield of 6.90%), respectively. Among these compounds, -cyhalothrin showed the strongest inhibitory effect against turnip peroxidase (K-i: 1.23 x 10(-2)+/- 0.21 x 10(-2)mM) as noncompetitive inhibition. On the other hand, cypermethrin demonstrated the highest inhibition effect against black radish peroxidase (K-i: 2.14 x 10(-2)+/- 0.08 x 10(-2)mM) as competitive inhibition.