Alteration of nitric oxide production in rats exposed to a prolonged, extremely low-frequency magnetic field

Akdag M. Z. , Bilgin M. H. , Dasdag S. , Tumer C.

ELECTROMAGNETIC BIOLOGY AND MEDICINE, vol.26, pp.99-106, 2007 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 26
  • Publication Date: 2007
  • Doi Number: 10.1080/15368370701357866
  • Page Numbers: pp.99-106


The purpose of this stud), is to investigate the possible effect of an extremely low frequency magnetic field (ELF-MF) on nitric oxide (NO) level. In this study, 27 male Sprague-Dawley rats were used. The rats were divided into three groups: two experimental and one control (sham-exposed). The first and second experimental group (n = 10) were exposed to 100 mu T and 500 mu T ELF-MF during 10 months, 2 h a day, respectively, and the third (n = 7) group was treated like an experimental group except for ELF-MF exposure in methacrylate boxes. After ELF-MF and sham exposure, serum nitrite levels were measured by Griess reaction. A significant reduction was observed in nitrite levels among the first and second experimental groups of rats and sham-exposed rats after exposure for 10 months, 2 h a day, to ELF-MF of 100 and 500 LT (p < 0.01). These results suggest that prolonged ELF-MF exposure at intensities of exposure limits, determined by ICNIRP for public and occupational, may reduce NO production probably affected by NO generation pathways.