8. Uluslararası katılımlı Türk Toksikoloji Derneği Kongresi, Antalya, Turkey, 01 November 2012, pp.10-15
IN VITRO AND IN VIVO EVALUATION OF POTENTIAL ENDOCRINE DISRUPTING EFFECTS OF HERBAL DIETARY SUPPLEMENTS Hande Gurer-Orhan1, Yasemin Toker1, Duysal Uslu1, Ozlem Yilmaz-Dilsiz2, Altug Yavasoglu2, Erdal Bedir3 1Ege University, Faculty of Pharmacy, Department of Toxicology, 35100, Izmir, Turkey 2Ege University, Faculty of Medicine, Department of Histology & Embryology, 35100, Izmir, Turkey 3Ege University, Faculty of Engineering, Bioengineering Department, 35100, Izmir, Turkey Phytoestrogens are mostly known for their beneficial health effects however recent studies reported that they can be hazardous by exhibiting estrogenic activity at susceptible life stages and when administered late in life. Moreover herbal dietary supplements are self prescribed, get on the market without thorough toxicity testing and are not required to state the purity and amount of their active ingredients. The present study is undertaken to screen the potential endocrine disrupter (ED) effect of widely used herbal dietary supplements in Turkey; Astragalus Root Extract, Black Cohosh Root Extract, Dong Quai, Grape Seed Extract, Gotu Kola, Isoflavones, Saw Palmetto Berries, Spirulina, St. John’s Wort, Tribulus Terrestris and Valerian Root Extract. The potential ED effect of selected products is evaluated by a “receptor binding assay” which is suitable as a high-throughput screening assay for ER ligands. E-Screen assay is used as a second in vitro screening assay to evaluate whether the products are estrogenic or antiestrogenic. Among all tested products St. John’s Wort was found to have a potential to exhibit estrogenic activity. This possible effect of the product was further analyzed by the golden standard test for endocrine disrupters, uterotrophic assay in rats. Our results indicate that working with individual purified molecules rather than herbal extracts gives more accurate and more interpretable results. This study is supported by the Scientific and Technical Research Council of Turkey (TUBITAK) 108S202, by the Ege University Science and Technology Centre (EBILTEM) 09BIL033 and by Ege University Research Fund 11ECZ003 and 11ECZ007.