The effects of anax imperator adipokinetic hormone on the expression of brain-derived neurotrophic factor in a rat glioma cell line

Doğan S., Köktürk S., Mutlu O., Dağıstanlı F., Gelenli Dolanbay E., Usta E., ...More

MCM2019 14th MULTINATIONAL CONGRESS ON MICROSCOPY SEPTEMBER 15–20, 2019 IN BELGRADE, SERBIA, Belgrade, Serbia And Montenegro, 15 - 20 September 2019, pp.193-194

  • Publication Type: Conference Paper / Summary Text
  • City: Belgrade
  • Country: Serbia And Montenegro
  • Page Numbers: pp.193-194


bstract Objectives: Adipokinetic hormones (AKHs) are small stress peptides found in insects that regulate metabolic responses to stress (1). Insect anti-stress responses, including those induced by insecticides, are controlled by adipokinetic hormones. AKHs are synthesized by neurosecretory cells of the corpora cardiaca of insects. However, presence of AKHs had also been reported the cells within the brain, although their roles are not clear (2). Astrocytes have been shown to play crucial roles in regulating both normal and disease states. Astrocyte dysfunction has been related to neuroautoimmune diseases, neoplasms and epilepsy (3-5). Brain-derived neurotrophic factor (BDNF) is one of the major neurotrophic factors produced by astrocytes to maintain the development and survival of neurons in the brain, and have recently been shown to modulate homeostasis of neuroinflammation. BDNF plays vitally important roles in neural development and plasticity in both health and disease. Glial cells are able to store and release BDNF (6) and it has been suggested that glial dysfunction may contribute to the patho-physiology of schizophrenia (7-9). The aim of this study was to investigate the effects of Anax imperator AKH (Ani-AKH) on the astrocyte-like model C6 rat glioma cell line, using BDNF immunohistochemistry stainings. Methods: The C6 rat glioma cell line was cultured in Dulbecco's modified Eagle's medium-F12 cell culture medium supplemented with 10% heat-inactivated fetal calf serum. Ani-AKH was applied to the cultured cells at the concentrations of 5, 10 and 50 µg/ml for 24 hours and evaluated the BDNF staining density. Results: BDNF staining density was significantly increased in the Anax imperator AKH (AniAKH) 5, 10 and 50 µg/ml groups than the control group. BDNF staining density was higher in the 50 µg/ml Ani-AKH group than in the 5 and 10- µg/ml Ani-AKH groups. Discussion: Our findings suggest that Ani-AKH has a potential increased the expression of BDNF on C6 rat glioma cell line. The further studies will be required working with additional cell lines, different doses and experimental design